Cotton Rat CD4 Antibody

Catalog #: MAB6676 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB6676
MAB6676-SP

Detection of CD4 in Cotton Rat Splenocytes by Flow Cytometry.

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Cotton Rat CD4 Antibody Summary

Species Reactivity

Cotton Rat

Specificity

Detects cotton rat CD4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant CD4 from human, mouse, rat, cat, or dog is observed.

Source

Monoclonal Mouse IgG_2B Clone # 695542

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant cotton rat CD4
Lys26-Pro393

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD4 in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD4 Monoclonal Antibody (Catalog # MAB6676, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and the Th1, Th2, Th17, Tfh, and Treg subsets of mature T lymphocytes. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binding to MHC class II molecules on antigen presenting cells contributes to the formation of the immunological synapse. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4