Cotton Rat CCL3/MIP‑1 alpha Biotinylated Antibody

Catalog #: BAF1061 Datasheet

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BAF1061

All CCL3/MIP-1 alpha Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Cotton Rat CCL3/MIP‑1 alpha Biotinylated Antibody Summary

Species Reactivity

Cotton Rat

Specificity

Detects cotton rat CCL3/MIP‑1 alpha in Western blots. In this format, approximately 30% cross-reactivity with recombinant mouse CCL3/MIP‑1 alpha is observed and less than 1% cross-reactivity with recombinant human CCL3/MIP‑1 alpha and recombinant rat CCL3/MIP‑1 alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant cotton rat CCL3/MIP-1 alpha (R&D Systems, Catalog # 1061-RM)
Ala24-Ala92
Accession # AAL26704

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061-RM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

MIP-1 alpha is a beta family (CC) chemokine and has been designated CCL3. MIP-1 alpha and MIP-1 beta, two closely related but distinct proteins, were originally purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Cotton rat MIP-1 alpha cDNA encodes a 92 amino acid (aa) residue precursor protein with a 23 aa putative signal peptide. Mature cotton rat MIP-1 alpha shares approximately 70% amino acid identity with human MIP-1 alpha. MIP-1 alpha is expressed in a wide variety of cells, including lymphocytes, fibroblasts, and epithelial cells, as well as monocytes/macrophages.

MIP-1 alpha has been shown to play an important role in the recruitment of mononuclear cells. Additionally, MIP-1 alpha has been reported to have chemoattractant and adhesive effects on lymphocytes, preferentially promoting the chemotaxis of Th1 cells. MIP-1 alpha has also been shown to attract B cells, eosinophils, and dendritic cells. In addition, MIP-1 alpha augments cytolytic activity of NK cells (1). MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. It has been demonstrated that MIP-1 alpha can bind the chemokine receptors CCR1 and CCR5 (2).

References

Robertson, M. (2002) J. Leukoc. Biol. 71:173.
Zlotnik, A. et al. (2000) Immunity 12:121.

Entrez Gene IDs

6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)

Alternate Names

C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta