Cotton Rat CCL3/MIP-1 alpha Antibody

Catalog #: AF1061 Datasheet

Catalog #	Availability	Size / Price	Qty
AF1061
AF1061-SP

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutral-ization by Cotton Rat CCL3/MIP‑1 alpha Antibody.

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Cotton Rat CCL3/MIP-1 alpha Antibody Summary

Species Reactivity

Cotton Rat

Specificity

Detects cotton rat CCL3/MIP‑1 alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MIP-1 alpha is observed, 15% cross-reactivity with recombinant human (rh) MIP-1 alpha is observed, less than 10% cross-reactivity with rhMIP‑1 beta, rh6Ckine, and rhHCC-4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant cotton rat CCL3/MIP-1 alpha
Ala24-Ala92
Accession # AAL26704

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061-RM)

Neutralization

Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 10 ng/mL Recombinant Cotton Rat CCL3/MIP‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutral-ization by Cotton Rat CCL3/MIP‑1 alpha Antibody. Recombinant Cotton Rat CCL3/MIP-1a (Catalog # 1061-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1061). The ND₅₀ is typically 0.1-0.4 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

MIP-1 alpha is a beta family (CC) chemokine and has been designated CCL3. MIP-1 alpha and MIP-1 beta, two closely related but distinct proteins, were originally purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Cotton rat MIP-1 alpha cDNA encodes a 92 amino acid (aa) residue precursor protein with a 23 aa putative signal peptide. Mature cotton rat MIP-1 alpha shares approximately 70% amino acid identity with human MIP-1 alpha. MIP-1 alpha is expressed in a wide variety of cells, including lymphocytes, fibroblasts, and epithelial cells, as well as monocytes/macrophages.

MIP-1 alpha has been shown to play an important role in the recruitment of mononuclear cells. Additionally, MIP-1 alpha has been reported to have chemoattractant and adhesive effects on lymphocytes, preferentially promoting the chemotaxis of Th1 cells. MIP-1 alpha has also been shown to attract B cells, eosinophils, and dendritic cells. In addition, MIP-1 alpha augments cytolytic activity of NK cells (1). MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. It has been demonstrated that MIP-1 alpha can bind the chemokine receptors CCR1 and CCR5 (2).

References

Robertson, M. (2002) J. Leukoc. Biol. 71:173.
Zlotnik, A. et al. (2000) Immunity 12:121.

Entrez Gene IDs

6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)

Alternate Names

C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta