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CometAssay Electrophoresis Starter Kit

Catalog #: 4250-050-ESK
25 Citations Datasheet
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4250-050-ESK
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Citations (25)
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CometAssay Electrophoresis Starter Kit Summary

Provides the reagents and instrumentation to standardize the Comet assay.
 

Key Benefits

• Specially treated slides to promote agarose adherence
• Ready to use low melt agarose (LMAgarose) in convenient size
• Lysis Solution suitable for either alkaline or neutral comet assay
• Optimized electrophoresis conditions

What is the CometAssay?

The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.

Why Use the CometAssay Electrophoresis Starter Kit?

CometAssay Electrophoresis Starter Kit offers a complete standardized system for the comet assay which includes a 50 test sample Reagent Kit (25 x 2 well slides), Alkaline Control Cells, and the CometAssay Electrophoresis System II for consistent and reproducible results. Each lot of the Alkaline Control Cells, reagents and CometSlides are tested and qualified for use in the CometAssay ES II.

Kit Contents

• CometAssay Electrophoresis System
• CometAssay Lysis Solution
• CometAssay LMAgarose
• CometSlides (2 Well, 25 Slides)
• 200 mM EDTA, pH 10
• CometAssay Alkaline Control Cells
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

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Citations for CometAssay Electrophoresis Starter Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. SRCAP mutations drive clonal hematopoiesis through epigenetic and DNA repair dysregulation
    Authors: Chen, CW;Zhang, L;Dutta, R;Niroula, A;Miller, PG;Gibson, CJ;Bick, AG;Reyes, JM;Lee, YT;Tovy, A;Gu, T;Waldvogel, S;Chen, YH;Venters, BJ;Estève, PO;Pradhan, S;Keogh, MC;Natarajan, P;Takahashi, K;Sperling, AS;Goodell, MA;
    Cell stem cell  2023-10-16
  2. MiR-497-3p induces Premature ovarian failure by targeting KLF4 to inactivate Klotho/PI3K/AKT/mTOR signaling pathway
    Authors: Zhou, Y;Yuan, F;Jia, C;Chen, F;Li, F;Wang, L;
    Cytokine  2023-08-05
  3. Lead induces mouse skin fibroblast apoptosis by disrupting intracellular homeostasis
    Authors: Wang, H;Wang, H;Guan, J;Guan, W;Liu, Z;
    Scientific reports  2023-06-14
  4. STC2 activates PRMT5 to induce radioresistance through DNA damage repair and ferroptosis pathways in esophageal squamous cell carcinoma
    Authors: K Jiang, X Yin, Q Zhang, J Yin, Q Tang, M Xu, L Wu, Y Shen, Z Zhou, H Yu, S Yan
    Redox Biology, 2023-02-03;60(0):102626.  2023-02-03
  5. Health risk assessment of cadmium exposure by integration of an in silico physiologically based toxicokinetic model and in vitro tests
    Authors: Y Zhang, Z Liu, Z Wang, H Gao, Y Wang, M Cui, H Peng, Y Xiao, Y Jin, D Yu, W Chen, Q Wang
    Journal of Hazardous Materials, 2022-10-14;443(0):130191.  2022-10-14
  6. Regulation of nuclear DNA damage response by mitochondrial morphofunctional pathway
    Authors: NTK Oanh, HS Lee, YH Kim, S Min, YJ Park, J Heo, YY Park, WC Lim, H Cho
    Nucleic Acids Research, 2022-09-09;0(0):.  2022-09-09
  7. The KU-PARP14 axis differentially regulates DNA resection at stalled replication forks by MRE11 and EXO1
    Authors: A Dhoonmoon, CM Nicolae, GL Moldovan
    Nature Communications, 2022-08-27;13(1):5063.  2022-08-27
  8. Panicle Apical Abortion 7 Regulates Panicle Development in Rice (Oryza sativa L.)
    Authors: D Dai, H Zhang, L He, J Chen, C Du, M Liang, M Zhang, H Wang, L Ma
    International Journal of Molecular Sciences, 2022-08-22;23(16):.  2022-08-22
  9. CometAnalyser: A user-friendly, open-source deep-learning microscopy tool for quantitative comet assay analysis
    Authors: A Beleon, S Pignatta, C Arienti, A Carbonaro, P Horvath, G Martinelli, G Castellani, A Tesei, F Piccinini
    Computational and structural biotechnology journal, 2022-08-03;20(0):4122-4130.  2022-08-03
  10. TOP1-DNA trapping by exatecan and combination therapy with ATR inhibitor
    Authors: U Jo, Y Murai, KK Agama, Y Sun, LK Saha, X Yang, Y Arakawa, S Gayle, K Jones, V Paralkar, RK Sundaram, J Van Doorn, JC Vasquez, RS Bindra, WS Choi, Y Pommier
    Molecular Cancer Therapeutics, 2022-07-05;0(0):.  2022-07-05
  11. A proteogenomic analysis of clear cell renal cell carcinoma in a Chinese population
    Authors: Y Qu, J Feng, X Wu, L Bai, W Xu, L Zhu, Y Liu, F Xu, X Zhang, G Yang, J Lv, X Chen, GH Shi, HK Wang, DL Cao, H Xiang, L Li, S Tan, HL Gan, MH Sun, J Qiu, H Zhang, JY Zhao, D Ye, C Ding
    Nature Communications, 2022-04-19;13(1):2052.  2022-04-19
  12. Proteomes of primary skin fibroblasts from healthy individuals reveal altered cell responses across the life span
    Authors: D Tsitsipati, JL Martindale, C Ubaida-Moh, A Lyashkov, H Yanai, A Kashyap, CH Shin, AB Herman, E Ji, JH Yang, R Munk, C Dunn, Y Lukyanenko, X Yang, CW Chia, AC Karikkinet, L Zukley, J D'Agostino, M Kaileh, CY Cui, I Beerman, L Ferrucci, M Gorospe
    Aging Cell, 2022-04-15;0(0):e13609.  2022-04-15
  13. Apoptotic and DNA Damage Effect of 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose in Cisplatin-Resistant Non-Small Lung Cancer Cells via Phosphorylation of H2AX, CHK2 and p53
    Authors: JH Kim, E Im, J Lee, HJ Lee, DY Sim, JE Park, CH Ahn, HH Kwon, BS Shim, B Kim, SH Kim
    Cells, 2022-04-14;11(8):.  2022-04-14
  14. HuR Plays a Role in Double-Strand Break Repair in Pancreatic Cancer Cells and Regulates Functional BRCA1-Associated-Ring-Domain-1(BARD1) Isoforms
    Authors: A Jain, M McCoy, C Coats, SZ Brown, S Addya, C Pelz, RC Sears, CJ Yeo, JR Brody
    Cancers, 2022-04-06;14(7):.  2022-04-06
  15. HspBP1 is a dual function regulatory protein that controls both DNA repair and apoptosis in breast cancer cells
    Authors: CK Youn, JH Lee, G Hariharasu, HB Kim, J Kim, S Lee, SC Lim, SP Yoon, SG Park, IY Chang, HJ You
    Cell Death & Disease, 2022-04-06;13(4):309.  2022-04-06
  16. Effective targeting of breast cancer stem cells by combined inhibition of Sam68 and Rad51
    Authors: A Turdo, M Gaggianesi, S Di Franco, V Veschi, C D'Accardo, G Porcelli, M Lo Iacono, I Pillitteri, F Verona, G Militello, A Zippo, V Poli, L Fagnocchi, S Beyes, S Stella, R Lattanzio, N Faldetta, VL Lentini, R Porcasi, G Pistone, MR Bongiorno, G Stassi, R De Maria, M Todaro
    Oncogene, 2022-02-25;0(0):.  2022-02-25
  17. TOPORS-mediated RAD51 SUMOylation facilitates homologous recombination repair
    Authors: G Hariharasu, SY Jeong, MJ Kim, SM Jung, G Seo, JR Moon, S Lee, IY Chang, Y Kee, HJ You, JH Lee
    Nucleic Acids Research, 2022-02-22;0(0):.  2022-02-22
  18. The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling
    Authors: PA Yao, Y Wu, K Zhao, Y Li, J Cao, C Xing
    Cell Death & Disease, 2022-02-02;13(2):103.  2022-02-02
  19. The BCL-2 family member BOK promotes KRAS-driven lung cancer progression in a p53-dependent manner
    Authors: AL Meinhardt, E Munkhbaata, U Höckendorf, M Dietzen, M Dechant, M Anton, A Jacob, K Steiger, W Weichert, L Brcic, N McGranahan, C Branca, T Kaufmann, MA Dengler, PJ Jost
    Oncogene, 2022-01-29;41(9):1376-1382.  2022-01-29
  20. Loss of MED12 activates the TGFbeta pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells
    Authors: LM Jackson, A Dhoonmoon, A Hale, KA Dennis, EM Schleicher, CM Nicolae, GL Moldovan
    Nucleic Acids Research, 2021-12-16;49(22):12855-12869.  2021-12-16
  21. MLK4 regulates DNA damage response and promotes triple-negative breast cancer chemoresistance
    Authors: D Mehlich, M ?omiak, A Sobiborowi, A Mazan, D Dymerska, ?M Szewczyk, A Mehlich, A Borowiec, MK Pre?owska, A Gorczy?ski, P Jab?o?ski, E I?ycka-?wi, D Nowis, AA Marusiak
    Cell Death & Disease, 2021-11-27;12(12):1111.  2021-11-27
  22. Airway Exposure to Polyethyleneimine Nanoparticles Induces Type 2 Immunity by a Mechanism Involving Oxidative Stress and ATP Release
    Authors: Y Srisomboon, N Ohkura, K Iijima, T Kobayashi, PJ Maniak, H Kita, SM O'Grady
    International Journal of Molecular Sciences, 2021-08-23;22(16):.  2021-08-23
  23. DCAF14 promotes stalled fork stability to maintain genome integrity
    Authors: A Townsend, G Lora, J Engel, N Tirado-Cla, H Dungrawala
    Cell Reports, 2021-01-26;34(4):108669.  2021-01-26
  24. The Antioxidant from Ethanolic Extract of Rosa cymosa Fruits Activates Phosphatase and Tensin Homolog In Vitro and In Vivo: A New Insight on Its Antileukemic Effect
    Authors: KC Wang, YC Liu, M El-Shazly, SP Shih, YC Du, YM Hsu, HY Lin, YC Chen, YC Wu, SC Yang, MC Lu
    Int J Mol Sci, 2019-04-19;20(8):.  2019-04-19
  25. FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress.
    Authors: Jeong Y, Rossi M, Cermak L, Saraf A, Florens L, Washburn M, Sung P, Schildkraut C, Pagano M
    J Cell Biol, 2013-01-14;200(2):141-9.  2013-01-14

FAQs

  1. Will any commercial horizontal gel electrophoresis device work for the Comet Assay?

    • Electrophoresis conditions described for FLARE™ Assay, CometAssay® and CometChip® Kits were optimized for the CometAssay® Electrophoresis System (Catalog # 4250-050-ES). Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). They can be used, but they require optimization by the user to achieve consistent results.

  2. What power supply is recommended for the CometAssay Electrophoresis System?

    • We recommend PowerPac™ HC High-Current Power Supply (1645052), available from Bio-Rad.

  3. Which adaptor can be used with the PowerPac ( Bio-Rad®, Catalog # 1645052) recommended for the CometAsay Electrophoresis System II?

    • The PowerPac Adator (Catalog # 1645064) can be used with the PowerPac recommended for the CometAsay Electrophoresis System II.

  4. Is the Comet Analysis software included in the Starter Kit Catalog # 4250-050-ESK? 

    • The software is not included with catalog # 4250-050-ESK. The software is available for purchase as catalog # 4260-000-CS.

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