Canine IL-4 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: IL-4
IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.
Citations for Canine IL-4 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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MicroRNA-194 regulates parasitic load and IL-1?-dependent nitric oxide production in the peripheral blood mononuclear cells of dogs with leishmaniasis
Authors: Costa, SF;Soares, MF;Poleto Bragato, J;Dos Santos, MO;Rebech, GT;de Freitas, JH;de Lima, VMF;
PLoS neglected tropical diseases
Species: Canine
Sample Types: Cell Culture Supernates
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DNA Vaccine Co-Expressing Hemagglutinin and IFN-? Provides Partial Protection to Ferrets against Lethal Challenge with Canine Distemper Virus
Authors: Zhao, J;Sun, Y;Sui, P;Pan, H;Shi, Y;Chen, J;Zhang, H;Wang, X;Tao, R;Liu, M;Sun, D;Zheng, J;
Viruses
Species: Ferret
Sample Types: Serum
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Immortalised canine buccal epithelial cells' CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol
Authors: M Pelst, C Höbart, H de Rooster, B Devriendt, E Cox
Veterinary research, 2022-09-13;53(1):72.
Species: Canine
Sample Types: Cell Culture Supernates
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Mycobacterium intracellulare induces a Th17 immune response via M1-like macrophage polarization in canine peripheral blood mononuclear cells
Authors: S Kim, YS Hyun, HT Park, MK Shin, HS Yoo
Scientific Reports, 2022-07-12;12(1):11818.
Species: Canine
Sample Types: Cell Culture Supernates
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Combined IMIG and Immune Ig Attenuate Allergic Responses in Beagle Dogs
Authors: RM Gorczynski, T Maqbool, G Hoffmann
J Immunol Res, 2020-04-21;2020(0):2061609.
Species: Canine
Sample Types: Cell Culture Supernates
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A preliminary study assessing cytokine production following ex vivo stimulation of whole blood with diet in dogs with chronic enteropathy
Authors: A Kathrani, E Hall
BMC Vet. Res., 2019-06-04;15(1):185.
Species: Canine
Sample Types: Cell Culture Supernates
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Neutrophil properties in healthy and Leishmania infantum-naturally infected dogs
Authors: AB Wardini, LH Pinto-da-S, NR Nadaes, MT Nascimento, BM Roatt, AB Reis, KF Viana, RC Giunchetti, EM Saraiva
Sci Rep, 2019-04-18;9(1):6247.
Species: Canine
Sample Types: Cell Culture Supernates
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PD-1 and PD-L1 regulate cellular immunity in canine visceral leishmaniasis
Authors: KL Oliveira S, V Marin Chik, G Luvizotto, AA Correa Lea, BF de Almeida, F De Rezende, PSP Dos Santos, G Fabrino Ma, VMF De Lima
Comp. Immunol. Microbiol. Infect. Dis., 2018-12-14;62(0):76-87.
Species: Canine
Sample Types: Cell Culture Supernates
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Oat beta-glucan as a dietary supplement for dogs
Authors: LG Ferreira, M Endrighi, KG Lisenko, MRD de Oliveir, MR Damasceno, JA Claudino, PG Gutierres, AP Peconick, FMOB Saad, MG Zangeronim
PLoS ONE, 2018-07-31;13(7):e0201133.
Species: Canine
Sample Types: Plasma
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Expression of regulatory T cells in jejunum, colon, and cervical and mesenteric lymph nodes of dogs naturally infected with Leishmania infantum.
Authors: Figueiredo M, Deoti B, Amorim I, Pinto A, Moraes A, Carvalho C, da Silva S, de Assis A, de Faria A, Tafuri W
Infect Immun, 2014-06-16;82(9):3704-12.
Species: Canine
Sample Types: Tissue Homogenates
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