Canine IL-17 Antibody

Catalog #: MAB5848 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB5848
MAB5848-SP

IL-6 Secretion Induced by IL‑17 and Neutralization by Canine IL‑17 Antibody.

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All IL-17/IL-17A Antibodies

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Canine IL-17 Antibody Summary

Species Reactivity

Canine

Specificity

Detects canine IL-17 in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) IL-17A is observed and no cross-reactivity with rhIL-17F or recombinant mouse IL-17A is observed.

Source

Monoclonal Mouse IgG_2A Clone # 665909

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant canine IL-17
Gly26-Ala155
Accession # NP_001159350

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND₅₀) is typically 5-30 ng/mL in the presence of 5 ng/mL Recombinant Canine IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IL-6 Secretion Induced by IL‑17 and Neutralization by Canine IL‑17 Antibody. Recombinant Canine IL-17 (Catalog # 5848-CL) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). IL-6 Secretion elicited by Recombinant Canine IL-17 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-17 Monoclonal Antibody (Catalog # MAB5848). The ND₅₀ is typically 5-30 ng/mL.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin 17 (IL-17; also IL-17A and CTLA-8) is a 17 kDa member of the IL-17 family of cytokines (1). Members of this family demonstrate a structural motif termed a cysteine knot which characterize a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (2-4). Based on the amino acid (aa) sequence alignment with human IL-17, canine IL-17 is 130 aa in length. It is secreted as a 35 kDa disulfide-linked homodimer and as a 40 kDa disulfide-linked heterodimer with IL-17F (5). Canine IL‑17 is 81% identical on the aa level to human IL-17. IL-23 drives Th17 lymphocytes to produce IL-17 (6-8). IL-17’s production has also been demonstrated in gamma δ T cells (9), CD8⁺ memory T cells (10-11), eosinophils (12), neutrophils (10), and monocytes (13). Studies have identified that the widely expressed receptors IL‑17RA and IL-17RC form a heterodimer for the binding of IL-17 (6, 14-15). The predominant function of IL-17 is thought to be as a proinflammatory mediator through a variety of mechanisms (16). Locally, IL‑17 stimulates production of IL-6, prostaglandin E and nitric oxide (16-19), and synergy with other inflammatory cytokines such as TNF-alpha, IL‑1 beta and IFN -gamma leads to up-regulation of gene expression and progression and amplification of local inflammation (16, 20-22). IL‑17 also mediates chemotaxis of neutrophils and monocytes to sites of inflammation through the chemoattractant mediators IL-8, GRO‑ alpha, and MCP-1 (16, 22-25) while augmenting production of hematopoietic growth factors, such as G-CSF and GM-CSF (16, 26, 27), which promote the growth and maturation of the recruited myeloid cells. In addition, IL-17 serves as a bridge between innate and adaptive immune responses by enhancing the induction of co-stimulatory molecules such as ICAM-1 and other cytokines (16, 22, 28), thereby supporting T cell activation. IL-17 expression has been associated with many inflammatory diseases, such as rheumatoid arthritis, multiple sclerosis, asthma, systemic lupus erythematosus and allograft rejection (15).

References

Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
Kolls, J.K. and A. Linden (2004) Immunity 21:467.
Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
Steinman, L. (2007) Nat. Med. 13:139.
Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
Lockhart, E. et al. (2006) J. Immunol. 177:4662.
Ferretti, S. et al. (2003) J. Immunol. 170:2106.
Shin, H.C. et al. (1999) Cytokine 11:257.
Molet, S. et al. (2001) J. Allergy Clin. Immunol. 108:430.
Zhou, Q. et al. (2005) Infect. Immun. 73:935.
Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
Yao, Z. et al. (1995) Immunity 3:811.
Attur, M.G. et al. (1997) Arthritis Rheum. 40:1050.
Ruddy, M.J. et al. (2004) J. Biol. Chem. 279:2559.
Albanesi, C. et al. (1999) J. Immunol. 162:494.
Witowski, J. et al. (2000) J. Immunol. 165:5814.
Miyamoto, M. et al. (2003) J. Immunol. 170:4665.
Ye, P. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:335.
Laan, M. et al. (2001) Br. J. Pharmacol. 133:200.
Starnes, T. et al. (2002) J. Immunol. 169:642.
Jones, C.E. et al. (2002) Am. J. Respir. Cell Mol. Biol. 26:748.
Yao, Z. et al. (1995) J. Immunol. 155:5483.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A