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Canine IL-10 Antibody

Catalog #: MAB7352
2 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB7352
MAB7352-SP
Cell Proliferation Induced by IL‑10 and Neutralization by Canine IL‑10 Antibody.
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Citations (2)
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Canine IL-10 Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine IL-10 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 138128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant canine IL‑10
Ser20-Ile179
Accession # XP_855560
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Canine IL‑10 (Catalog # 735-CL)
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.1-0.25 µg/mL in the presence of 2 ng/mL Recombinant Canine IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Canine IL‑10 Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Canine IL‑10 Antibody. Recombinant Canine IL‑10 (Catalog # 735-CL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL‑10 Monoclonal Antibody (Catalog # MAB7352). The ND50 is typically 0.1-0.25 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones. The canine homologue of mouse IL-10 has been cloned. Canine IL-10 shares 80% amino acid sequence homology with human IL-10 and 72% sequence homology with mouse IL-10.

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

References
  1. Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Canine IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant
    Vaccine, 2016-09-21;0(0):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
    Authors: Figueiredo M, Amorim I, Pinto A, Barbosa V, Pinheiro L, Deoti B, Faria A, Tafuri W
    BMC Immunol, 2013-05-14;14(0):22.
    Species: Canine
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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