Canine IL-10 Antibody

Catalog #: MAB7351
4 Citations Datasheet

Catalog #	Availability	Size / Price	Qty
MAB7351
MAB7351-SP

All IL-10 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Canine IL-10 Antibody Summary

Species Reactivity

Canine

Specificity

Delects canine IL-10 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant feline IL-10, recombinant human (rh) IL‑10, recombinant mouse (rm) IL-10, recombinant porcine IL-10, rhIL-10 sR, rmIL-10 sR, or recombinant rat IL-10.

Source

Monoclonal Mouse IgG₁ Clone # 138111

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant canine IL‑10
Ser20-Ile179
Accession # XP_855560

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Canine IL-10 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Canine IL‑10 Biotinylated Antibody (Catalog # BAF735)

Standard: Recombinant Canine IL-10 Protein (Catalog # 735-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones. The canine homologue of mouse IL-10 has been cloned. Canine IL-10 shares 80% amino acid sequence homology with human IL-10 and 72% sequence homology with mouse IL-10. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

References

Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Canine IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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A Vaccine Therapy for Canine Visceral Leishmaniasis Promoted Significant Improvement of Clinical and Immune Status with Reduction in Parasite Burden
Authors: BM Roatt, RD Aguiar-Soa, LE Reis, JM Cardoso, FA Mathias, RC de Brito, SM da Silva, NF Gontijo, SA Ferreira, JG Valenzuela, R Corrêa-Oli, RC Giunchetti, AB Reis
Front Immunol, 2017-03-07;8(0):217.
Species: Canine
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs
PLoS Negl Trop Dis, 2016-05-25;10(5):e0004614.
Species: Canine
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function.
Authors: Esch K, Juelsgaard R, Martinez P, Jones D, Petersen C
J Immunol, 2013-10-23;191(11):5542-50.
Species: Canine
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization
Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
Authors: Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM
Vaccine, 2005-08-15;24(4):501-9.
Species: Canine
Sample Types: Cell Culture Supernates
Applications: ELISA Development