Canine/Feline SCF/c-kit Ligand Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB2268G Datasheet
Catalog # Availability Size / Price Qty
FAB2268G-100UG
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Canine/Feline SCF/c-kit Ligand Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Canine, Feline
Specificity
Detects canine and feline SCF/c‑kit Ligand in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50%‑100% cross‑reactivity with recombinant human (rh) SCF and recombinant mouse SCF is observed and no cross-reacti
Source
Monoclonal Mouse IgG1 Clone # 371907
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant canine SCF/c‑kit Ligand
Lys26-Ala190
Accession # Q06220
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SCF/c-kit Ligand

SCF, also known as c-kit ligand, is a 28 kDa‑40 kDa type I transmembrane (TM) glycoprotein that plays important roles in fetal and adult developmental processes. Endothelial cells, fibroblasts, and keratinocytes can express SCF. The 190 aa extracellular region contains a proteolytic cleavage site, generating 165 aa circulating monomers and noncovalent homodimers. Soluble feline and canine SCF share 93% aa identity with each other and 88%‑92% aa identity with pig, cow, sheep, goat, horse, and mink SCF.

Long Name
Stem Cell Factor
Entrez Gene IDs
4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
Alternate Names
c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor

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