Canine CD4 PE-conjugated Antibody

Catalog #: FAB2410P Datasheet

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FAB2410P

Detection of CD4 in Lymphocytes in Canine PBMCs by Flow Cytometry.

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Canine CD4 PE-conjugated Antibody Summary

Species Reactivity

Canine

Specificity

Detects canine CD4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD4, recombinant mouse CD4, and recombinant feline CD4 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 296712

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant canine CD4
Val25-Lys401
Accession # P33705

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD4 in Lymphocytes in Canine PBMCs by Flow Cytometry. Lymphocytes in canine peripheral blood mononuclear cells were stained with Mouse Anti-Canine CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB2410P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD4

CD4 is a 56-58 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed on a variety of cell types including T helper cells, follicular helper cells, neurons, T regulatory cells, microglia, and monocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV. Over aa 25-401, canine CD4 shares 56% amino acid identity with human CD4.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4