Canine CD25/IL-2R alpha Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c). IL‑2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 20 aa signal peptide, a 217 aa extracellular domain (ECD) with two Sushi domains, a 19 aa transmembrane segment, and an 12 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49% - 60% aa sequence identity with human, mouse, and rat IL-2 R alpha. IL-2 R beta (CD122) and gamma _c (IL‑2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma _c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2 R alpha are found in some cancers and immune disorders (10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (11‑13).

Product Datasheets

Related Research Areas

• Acute Lymphoblastic Leukemia
• Acute Myeloid Leukemia
• B Cell Activation
• B Cell Markers
• Cancer Biomarkers
• Common gamma Chain Receptor Family
• Cytokines and Receptors in Allergy and Asthma
• Cytokines and Receptors in Tfh Cell Differentiation and Function
• Group 1 ILCs
• Group 2 ILCs
• Isolation, Culture, and Identification of Natural Killer Cells
• LTi Cells
• Lymphoma
• NCR+ILC3
• NCR-ILC3
• Preeclampsia
• Receptors in the Jak/STAT Pathway
• Regulatory T Cells (Tregs)
• T Cell Cytokine Signaling