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Bovine Osteopontin (OPN) Protein

Catalog #: 109-OP
2 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
109-OP-050/CF

With Carrier

Catalog # Availability Size / Price Qty
109-OP-050
Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Bovine Osteopontin (OPN) Protein Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Hu, D.D. et al. (1995) J. Biol. Chem. 270:26232. When 1 x 105 cells/well are added to a Bovine Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C.  The ED50  for this effect is 0.06-0.36 µg/mL.
Source
Bovine milk-derived Osteopontin/OPN protein
SDS-PAGE
60 kDa, reducing conditions

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109-OP (with carrier)

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109-OP/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

109-OP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

109-OP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteopontin/OPN

Osteopontin (OPN, also known as early T lymphocyte activation protein 1, bone sialoprotein 1, and secreted phosphoprotein 1), is a secreted, highly acidic,
calcium-binding, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels, and various tumor tissues. Many cell types can express OPN in response to activation by cytokines, growth factors, or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, tumor metastasis, and cerebral ischemia (1, 2). Bovine OPN cDNA encodes a 278 amino acid (aa) residue precursor with a 16 aa signal peptide that is cleaved to yield a 262 aa mature protein with an integrin binding sequence (RGD), a thrombin cleavage site and N- and O-glycosylation sites. Human, mouse, rat, porcine, and bovine OPN share approximately 40%-80% aa sequence identity. OPN has been shown to bind to different cell types through RGD-mediated interaction with Integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and Integrins alpha 8 beta 1 or alpha 9 beta 1. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3, and MMP-7. The functions of OPN in a variety of cell types are modified as a result of proteolytic cleavage (3, 4).

References
  1. Sodek, J. et al. (2006) J. Dent. Res. 85:404.
  2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
  3. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  4. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

Citations for Bovine Osteopontin (OPN) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Paper-based aptasensor for colorimetric detection of osteopontin
    Authors: AC Pereira, FTC Moreira, LR Rodrigues, MGF Sales
    Analytica chimica acta, 2022-01-29;1198(0):339557.
    Species: Bovine
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  2. Facile coupling of synthetic peptides and peptide-polymer conjugates to cartilage via transglutaminase enzyme.
    Authors: Jones ME, Messersmith PB
    Biomaterials, 2007-09-17;28(35):5215-24.
    Species: Bovine
    Sample Types: Extracellular Matrix
    Applications: Binding Assay

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