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Bovine Fibronectin Protein, CF

Catalog #: 1030-FN
15 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
1030-FN-01M
1030-FN-05M
Staining of dopaminergic neurons with anti-tyrosine hydroxylase in green, anti-neuron-specific beta III tubulin Tuj1 in red and DAPI in blue
3 Images
Product Details
Citations (15)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Bovine Fibronectin Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is
1‑5 µg/cm2.  Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 µg/mL.
Optimal concentration depends on cell type as well as the application or research objectives. Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is 10.0-100 ng/mL.
Source
Bovine plasma-derived Fibronectin protein

Product Datasheets

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1030-FN

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1030-FN

Formulation Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Thaw frozen material overnight at 2-8 °C. Do not disturb the product during the initial thaw event. Avoid vortexing and excessive agitation of the product.

Scientific Data

Cell Culture Staining of dopaminergic neurons with anti-tyrosine hydroxylase in green, anti-neuron-specific beta III tubulin Tuj1 in red and DAPI in blue View Larger

Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red) and counterstained with DAPI (5748; blue).

Cell Culture Staining of dopaminergic neurons with anti-tyrosine hydroxylase in red, anti-neuron-specific beta III tubulin Tuj1 in green and DAPI in blue View Larger

Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively), and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (464-SH). Tyrosine Hydroxylase was detected using a Mouse Anti-Human Tyrosine Hydroxylase Monoclonal Antibody (MAB7566). The cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red). Neuron-specific beta-III Tubulin was detected using a Mouse Anti-Neuron-specific beta-III Tubulin (Clone Tuj-1) Monoclonal Antibody (MAB1195) followed by the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green). Cells were counterstained with DAPI (5748; blue).

Cell Culture Staining of oligodendrocytes with anti-Oligodendrocyte Marker O4 in red and counterstained with DAPI in blue View Larger

D3 mouse embryonic stem cells were expanded in KO-ES Media supplemented with Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003), and a panel of growth factors for effective oligodendrocyte differentiation, including Recombinant Human FGF-basic, Recombinant Human EGF (236-EG), and Recombinant Human PDGF-AA (221-AA). Oligodendrocytes were detected using a Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (MAB1326). The cells were stained with the NorthernLights™-557 Affinity-purified Goat Anti-Mouse IgM Secondary Antibody (NL019; red). The nuclei were counterstained with DAPI (5748;blue).

Background: Fibronectin

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules (1). It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, Fibronectin is highly adhesive when on the surface (2). Polymerization of Fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), Fibronectin plays an important role in normal morphogenesis, including cell adhesion, migration, differentiation, and specific gene expression (3 - 6).

References
  1. Vaheri, A. et al. (1978) Biochem. Biophys. Acta. 516:1.
  2. Pytela, R. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5766.
  3. Danen, E.H. et al. (2001) J. Cell. Physiol. 189:1.
  4. Pereira, M. et al. (2001) Am. NY Acad. Sci. 936:438.
  5. Ruoslahti, E. (1999) Adv. Cancer Review 76:1.
  6. Romberger, D.J. (1997) Int. J. Biochem. Cell Biol. 29:939.
Entrez Gene IDs
2335 (Human); 280794 (Bovine)
Alternate Names
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Citations for Bovine Fibronectin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Endolysosomal TRPML1 channel regulates cancer cell migration by altering intracellular trafficking of E-cadherin and ?1-integrin
    Authors: Frey, N;Ouologuem, L;Blenninger, J;Siow, WX;Thorn-Seshold, J;Stöckl, J;Abrahamian, C;Fröhlich, T;Vollmar, AM;Grimm, C;Bartel, K;
    The Journal of biological chemistry
    Species: Human, Mouse
    Sample Types: Transfected Whole Cells, Whole Cells
    Applications: Bioassay
  2. Multiomics analyses reveal DARS1-AS1/YBX1-controlled posttranscriptional circuits promoting glioblastoma tumorigenesis/radioresistance
    Authors: Zheng, C;Wei, Y;Zhang, Q;Sun, M;Wang, Y;Hou, J;Zhang, P;Lv, X;Su, D;Jiang, Y;Gumin, J;Sahni, N;Hu, B;Wang, W;Chen, X;McGrail, DJ;Zhang, C;Huang, S;Xu, H;Chen, J;Lang, FF;Hu, J;Chen, Y;
    Science advances
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer
    Authors: M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka
    Oncology Letters, 2022-09-30;24(5):417.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Confocal Blood Flow Videomicroscopy of Thrombus Formation over Human Arteries and Local Targeting of P2X7
    Authors: P Marchese, M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Chiesa, O Alfieri, C Foglieni
    International Journal of Molecular Sciences, 2021-04-14;22(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  5. Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
    Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
    Cell Rep, 2020-05-05;31(5):107599.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Cell Culture
  6. In vivo monitoring of dynamic interaction between neutrophil and human umbilical cord blood-derived mesenchymal stem cell in mouse liver during sepsis
    Authors: SY Ahn, YS Maeng, YR Kim, YH Choe, HS Hwang, YM Hyun
    Stem Cell Res Ther, 2020-02-03;11(1):44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin
    Authors: N Boukelmoun, GS Chiu, A Kavelaars, CJ Heijnen
    Acta Neuropathol Commun, 2018-12-12;6(1):139.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
    Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
    J Neuroinflammation, 2018-08-13;15(1):226.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
    Authors: J Yang, C Wu, I Stefanescu, A Horowitz
    J Vis Exp, 2017-04-22;0(122):.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  10. FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells.
    Authors: Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R
    Stem Cells, 2014-03-01;32(3):770-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The threonine protease activity of testes-specific protease 50 (TSP50) is essential for its function in cell proliferation.
    Authors: Li YY, Bao YL, Song ZB, Sun LG, Wu P, Zhang Y, Fan C, Huang YX, Wu Y, Yu CL, Sun Y, Zheng LH, Wang GN, Li YX
    PLoS ONE, 2012-05-04;7(5):e35030.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  12. IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit.
    Authors: Sasaki K, Pardee AD, Okada H, Storkus WJ
    Eur. J. Immunol., 2008-10-01;38(10):2865-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
    Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
    Stem Cells, 2007-10-11;25(12):3261-70.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling.
    Authors: Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ
    Am. J. Respir. Cell Mol. Biol., 2007-08-02;38(1):95-104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004-09-17;152(2):159-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    • Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 

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Bovine Fibronectin Protein, CF
By Anonymous on 11/27/2020
Application: Binding assay/Protein-protein interaction