Home / FGF acidic/FGF1 / Bovine FGF acidic/FGF1 Antibody

Bovine FGF acidic/FGF1 Antibody

Catalog #: AB-32-NA
3 Citations Datasheet
Catalog # Availability Size / Price Qty
AB-32-NA
Cell Proliferation Induced by FGF acidic/FGF1 and Neutral-ization by Bovine FGF acidic/FGF1 Antibody.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Bovine FGF acidic/FGF1 Antibody Summary

Species Reactivity
Bovine
Specificity
Detects bovine FGF acidic/FGF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize both bovine FGF acidic/FGF1 and recombinant human (rh) FGF acidic/FGF1. In direct ELISAs, no cross‑reactivity with rhFGF-basic, rhFGF-4, -5, -6, -7 or -9 is observed. Neutralizes the biological activity of bovine FGF acidic/FGF1 and will also neutralize the biological activity of rhFGF acidic/FGF1, although 5 times the amount of IgG is required.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Bovine brain-derived FGF acidic/FGF1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Bovine FGF acidic/FGF1 (Catalog # 132-FA)
Neutralization
Measured by its ability to neutralize FGF acidic/FGF1-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 0.75 ng/mL Bovine FGF acidic/FGF1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by FGF acidic/FGF1 and Neutral-ization by Bovine FGF acidic/FGF1 Antibody. View Larger

Cell Proliferation Induced by FGF acidic/FGF1 and Neutral-ization by Bovine FGF acidic/FGF1 Antibody. Bovine FGF acidic/FGF1 (Catalog # 132-FA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF acidic/FGF1 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Bovine FGF acidic/FGF1 Polyclonal Antibody (Catalog # AB-32-NA). The ND50 is typically 1-3 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1‑3). Bovine FGF acidic shares 53% amino acid sequence identity with FGF basic and 11%‑31% with other bovine FGFs. It shares 92%, 91%, 88%, and 91% aa sequence identity with human, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (4). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (5). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryognesis thereby regulate FGF acidic bioactivity (6). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (7, 8). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (9). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (10‑12). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (13, 14). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (15).

References
  1. Gimenez-Gallego, G. et al. (1985) Science 230:1385.
  2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  4. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
  5. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
  6. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
  7. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
  8. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  9. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  10. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
  11. Imamura, T. et al. (1990) Science 249:1567.
  12. Wesche, J. et al. (2005) Biochemistry 44:6071.
  13. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
  14. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
  15. Bouleau, S. et al. (2005) Oncogene 24:7839.
Long Name
Fibroblast Growth Factor acidic
Entrez Gene IDs
2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
Alternate Names
AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1

Product Datasheets

You must select a language.

x
Product Datasheet

Citations for Bovine FGF acidic/FGF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. FGF1 protects neuroblastoma SH-SY5Y cells from p53-dependent apoptosis through an intracrine pathway regulated by FGF1 phosphorylation
    Authors: C Pirou, F Montazer-T, N Jah, E Delmas, C Lasbleiz, B Mignotte, F Renaud
    Cell Death Dis, 2017-08-31;8(8):e3023.
  2. FGF1 C-terminal domain and phosphorylation regulate intracrine FGF1 signaling for its neurotrophic and anti-apoptotic activities
    Authors: E Delmas, N Jah, C Pirou, S Bouleau, N Le Floch, J-L Vayssière et al.
    Cell Death & Disease
  3. Human FGF-1 gene transfer promotes the formation of collateral vessels and arterioles in ischemic muscles of hypercholesterolemic hamsters.
    Authors: Caron A, Michelet S, Caron A, Sordello S, Ivanov MA, Delaere P, Branellec D, Schwartz B, Emmanuel F
    J Gene Med, 2004-09-01;6(9):1033-45.
    Species: Hamster
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Bovine FGF acidic/FGF1 Antibody

There are currently no reviews for this product. Be the first to review Bovine FGF acidic/FGF1 Antibody and earn rewards!

Have you used Bovine FGF acidic/FGF1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review