Biotin-NAD+

Biological Activity

Biotin-NAD+ is a provides a convenient non-isotopic alternative to radiolabeled NAD⁺ for determination of IC₅₀ values for candidate PARP inhibitors and studies requiring this substrate. Biotinylated-NAD⁺ allows an indirect measure of PARP activity when biotin incorporation is detected using a conjugated-streptavidin detection system. Acts as a substrate for ADP-ribosylation. Can be used to label and purify biotinyl-ADP ribosylated proteins.

This product is a replacement for R&D Systems product 4670-500-01.

131 μg is supplied as 500 μl of a 0.25 mM solution in water.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Purification of a nitric oxide-stimulated ADP-ribosylated protein using biotinylated beta-nicotinamide adenine dinucleotide.
   Zhang et al.
   Biochemistry, 1993;32:2228
2. The Hsp90 machinery facilitates the transport of diphtheria toxin into human cells.
   Schuster et al.
   Sci.Rep., 2017;7:613
3. Antitumor activity of a pyrrole-imidazole polyamide.
   
   Proc Natl Acad Sci U S A, 2013-01-14;110(5):1863-8.
4. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
   CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
   Mol. Cell, 2017-05-18;66(4):503-516.e5.

Product Datasheets