BDY FL, SE
Chemical Name: (T-4)-[1-[3-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrol-2-yl-κN]-1-oxopropoxy]-2,5-pyrrolidinedionato]difluoroboron
Purity: ≥95%
Biological Activity
Key information: BDY FL, SE is a fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye supplied with an NHS ester reactive group for the labeling of primary amines. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes.Application: BDY FL, SE exhibits a narrow emission bandwidth and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy.
Properties and Photophysical Data: Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. Exhibits similar excitation and emission spectra to dye FITC. Excitation and emission maxima (λ) are 502 nM and 510 nM, respectively; quantum yield = 0.97; extinction coefficient = 80,000 M-1cm-1 (measured in TFE plus 0.1% TFA); A280 = 0.027.
For more information on how BDY FL, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
It is recommended to prepare stock solutions in DMSO.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY FL, SE
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 502 nm |
| λem | 510 nm |
| Extinction Coefficient (ε) | 80000 M-1cm-1 |
| Quantum Yield (φ) | 0.97 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.015 |
| Correction Factor 280 | 0.027 |
| Application | Confocal Microscopy, Fluorescence Polarization Assays and Two-Photon Excitation (TPE) Microscopy |
| Alternative For | FAM; Alexa Fluor 488; DyLight 488; Cy2 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Mitochondria are the target organelle of differentiation-inducing factor-3, an anti-tumor agent isolated from Dictyostelium discoideum.
Kubohara et al.
PLoS ONE, 2013;8:e72118
Product Datasheets
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