ALDH Detection Reagent - BAAA-DA New
Chemical Name: (T-4)-[N-(2,2-Diethoxyethyl)-5-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron
Purity: ≥95%
Biological Activity
Key information: ALDH Detection Reagent - BAAA-DA is a fluorescent substrate of aldehyde dehydrogenase (ALDH).Used for: can be used in flow cytometry to sort cancer stem cells with high ALDH levels, and to isolate human hematopoietic progenitor cells. Can be used with efflux inhibitors such as Verapamil (Cat. No. 0654), Probenecid (Cat. No. 4107) or 2-deoxy-D-glucose (Cat. No. 4515) to block multi-drug resistance efflux.
Application: flow cytometry, fluorescent-activated cell sorting (FACS).
Properties and Photophysical Data: BAAA-DA is converted under acidic conditions to BAAA. BAAA, cell-permeant, is converted by intracellular ALDH into carboxylate ion amino acetate (BAA) and used to identify cells with high ALDH activity. Excitation and emission maxima (λ) are 505 nm and 512 nm, respectively; extinction coefficient = 74,595 M-1cm-1.
Optical Data for ALDH Detection Reagent - BAAA-DA
| λabs | 505 nm |
|---|---|
| λem | 512 nm |
| Extinction Coefficient (ε) | 74595 M-1cm-1 |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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