5-Ph-IAA

Biological Activity

5-Ph-IAA is a selective and potent "Bump & Hole" TAG Degrader (DC_50,6h = 17.0 nM, T_1/2 = 62.3 min), for use within an auxin-inducible degron (AID2) system. 5-Ph-IAA is an analog (the “Bump”) of natural auxin that induces rapid degradation of a target protein tagged with mAID F-box protein (the "Hole" or mini AID), and recruits a mutant E3 ligase complex, OsTIR1^(F74G). The induced degradation is reversible 3 h after washout with fresh medium. 5-Ph-IAA shows rapid depletion of mAID-tagged nuclear and cytoplasmic proteins in proliferating and non-proliferating mammalian cells with no detectable basal degradation, lower cytotoxicity level, increased potency and faster degradation of the reporter in comparison with the conventional AID system. The AID2 system also works in budding yeast and fission yeast, and it can control protein expression in multiple mouse organs in vivo.

5-Ph-IAA-AM (Cat. No. 7893), an analog of 5-Ph-IAA, is also available.

Plasmid vectors for the AAV transfection and CRISPR-mediated knock-in of OsTIR1^F74G and mAID tag are available from Addgene.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets