4-Hydroxynonenal Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB3249V Datasheet
Catalog # Availability Size / Price Qty
FAB3249V-100UG
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4-Hydroxynonenal Alexa Fluor® 405-conjugated Antibody Summary

Specificity
Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.
Source
Monoclonal Mouse IgG2b Clone # 198960
Purification
Protein A or G purified
Immunogen
KLH-coupled 4-Hydroxynonenal
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 4-Hydroxynonenal

4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

Alternate Names
(2E)-4-Hydroxy-2-nonenal ; 2-Nonenal, 4-hydroxy-, (2E,4R)-; 4 HNE; 4Hydroxynonenal; 4-Hydroxynonenal; 4-hydroxy-trans-2-nonenal; HNE

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